Dr. Eric Finzi, a board certified dermasurgeon, began studying the effects of Botox injections on women with major depressive disorder in 2006. He injected Botox into the frowning muscles (located between the eyebrows) of 10 women (9 with depression, 1 with bipolar disorder.) After two months, Dr. Finzi found that all 9 of the depressed women were no longer showing signs of it, and even the woman with bipolar disorder experienced an improvement in her mood.